Larvicidal activity of individual and combined ethanolic extracts of Annona muricata (Soursop) seed and Piper betle (Betel) leaf against Aedes aegypti

RALPH MARU M. GRANDE, MAXINE DANIELLE A. BALMACEDA, MADELEINE THERESE T. TANEÑA, and HAROLD P. MEDIODIA
Philippine Science High School – Western Visayas Campus, Brgy. Bito-on, Jaro, Iloilo City 5000, Department of Science and Technology – Science Education Institute, Philippines

Abstract
The present study investigated the larvicidal activity of individual and combined ethanolic extracts of Annona muricata seed and Piper betle leaf against third and early fourth instar Aedes aegypti larvae. Larvicidal bioassays were done according to the World Health Organization 2005 guidelines for laboratory testing of mosquito larvicides. Individual larvicidal bioassays for A. muricata and P. betle exhibited LC 50 values of 16.45 ppm and 847.86 ppm, respectively. Between the two combined extract test ratios, the 70:30 (A. muricata:P. betle) ratio was more effective with an LC 50 of 116.51 ppm compared to the 152.16 ppm LC 50 value of the 50:50 (A. muricata:P. betle) ratio. Results reveal A. muricata extract in this study exhibits greater larvicidal effect compared to previous larvicidal studies using similar extract. A. muricata seed and P. betle leaf ethanolic extracts are effective larvicides both used individually and in combination against third and early fourth instar A. aegypti larvae.

Keywords: Annona muricata, Piper betle, Aedes aegypti, larvicidal bioassay, ethanolic