Evaluation of the antioxidant activity of Annona muricata (Guyabano) mediated silver nanoparticles and Annona muricata ethanolic leaf extract

ANN THERESE S. SOBRETODO, MICA ANTONIA D. JAEN, RICHLYN JOY S. VILLALOBOS, and RAMON ANGELO N. SINCO
Philippine Science High School – Western Visayas Campus, Brgy. Bito-on, Jaro, Iloilo City 5000, Department of Science and Technology – Science Education Institute, Philippines

Abstract
Silver nanoparticles (AgNPs) biosynthesized from various plant sources is an emerging material used for several applications. These may be used as antioxidants as several studies have focused on its ability to eliminate free radicals. Annona muricata (Guyabano) leaves are commonly known to possess such antioxidant properties due to its present bioactive compounds—thus becoming a suitable capping agent for AgNPs. The present study compared the antioxidant activity of A. muricata leaf extract, A. muricata mediated AgNPs, and commercially available AgNPs against ascorbic acid (positive control) and methanol (negative control) via DPPH Assay. Results show the leaf extract having the highest antioxidant activity after the positive control. This suggests that the plant extract can primarily be used as an antioxidant while the synthesized AgNPs do not contribute to its antioxidant property.

Keywords: Annona muricata, silver nanoparticles, antioxidant, DPPH Assay, biosynthesis