ANTIBACTERIAL EFFECTS OF CRUDE EXTRACTS FROM MAHINOT ESCULENTA TUBERS, ALLIUM SATIVUM RHIZOME, AND LYCOPERSICON ESCULANTUM BERRIES ON BACCILUS SUBTILIS, ESCHERCIA COLI AND STAPHYLOCOCCUS AUREUS

Edward C. Albaracin, Vincent Louie Delfin, Frederick Bedonia (January 1998)

Philippine Science High School Western Visayas Campus – Department of Science and Technology (DOST-PSHS WVC), Brgy. Bito-on, Jaro, Iloilo City 5000, Philippines

ABSTRACT

This study tests the potentials of Mahinot esculenta tubers, Allium sativum rhizome, and Lycopersicon esculantum berries of their anti-microbial effects on Staphylococcus aureus, Escherichia coli, and Bacillus subtilis.

Extracts of garlic, cassava tubers, and tomato berries were made by adding water to a 1:2 solution. The extracts were tested for their anti-microbial effects using the Filter Paper Disc-Agar Plate Method with millimeters as the unit of measurement. Random Block Analysis of Variance and Duncan’s multiple range test method at a 0.05 level of significance were used to test if there is a significant difference in the anti-bacterial effects of the extracts.

Results showed that garlic, cassava and tomato have anti-bacterial effects on the test organisms.

There is a significant difference on the zones of inhibition of the different extracts as anti-microbial agents on Bacillus subtilis, Escherichia coli, and Staphylococcus aureus. Antibacterial activity of the treatments on Escherichia coli decreased in this order Ciprofloxacin>Allium sativum rhizome >Mahinot esculenta tubers>Lypersicon esculantum berries>Distilled H20. Antibacterial activity of the treatments on Staphylococcus aureus decreased in this order Ciprofloxacin>Allium sativum rhizome >Mahinot esculenta tubers>Lypersicon esculantum berries>Distilled H20. Antibacterial activity of the treatments on Bacillus subtilis decreased in this order Ciprofloxacin>Allium sativum rhizome >Mahinot esculenta tubers>Lypersicon esculantum berries>Distilled H20.

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