ANTIBACTERIAL ACTIVITY SCREENING OF Chrysanthemum CRUDE EXTRACTS, DISTILLAND, AND ESSENTIAL OIL AGAINST Escherichia coli, Staphylococcus aureus, AND Bacillus subtilis

Collen April D. Celis, Lynn Rose M. Jabagat, Lorelyn May P. Natonton (March 2002)

Philippine Science High School Western Visayas Campus – Department of Science and Technology (DOST-PSHS WVC), Brgy. Bito-on, Jaro, Iloilo City 5000, Philippines

ABSTRACT

This study was conducted to determine the antibacterial activity of Chrysanthemum crude extracts, distilland, and essential oil against Escherichia coli, Staphylococcus aureus, and Bacillus subtilis. The method used in extracting the essential oil and crude extracts were steam distillation and hot water extraction respectively. Streaking of bacteria from the pure culture was applied to the antibacterial agents. The results were measured by counting the diameter of the zones of inhibition after 24 hours of incubation period.

The descriptive statistical tools used in this study are the mean and standard deviation while the inferential statistical tool used is the One-Way Analysis of Variance (ANOVA). The Scheffe test is used as post hoc multiple comparison test.

Results showed the except for the positive control, tetracycline, only the Chrysanthemum crude extracts exhibited antibacterial activities against the three test organisms but remains incomparable to the potency of tetracycline. Based on the statistical analyses of the results, the researchers recommend that Chrysanthemum crude extracts is a promising antibacterial drug against E coli.

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