Rich D. Dilla, Ma. Salvacion A. Valdellon (February 1999)
Philippine Science High School Western Visayas Campus – Department of Science and Technology (DOST-PSHS WVC), Brgy. Bito-on, Jaro, Iloilo City 5000, Philippines
ABSTRACT
This study tested the antibacterial effects of root extracts of botonsilyo (Cyperus rotundus), (Cyperus paragio (Mimosa pudica), the brevifolia), mutha (Eleusine indica), makahiya tagulinaw (Vernonia cinerea) and kandi-kandilaan (Stachytarpheta jamaicensis) against two pathogens: Escherichia coli (gram-negative) and Staphylococcus aureus (gram-positive) using the Filter Paper Disc- Agar Diffusion Method. Tetracycline, a commercial drug was used as positive control and 80% ethanol as negative control.
Roots were extracted by adding 75 ml of 80% ethanol to 25 grams of roots of each weed. were fluxed and concentrated to 10 ml. They Three replicates were made for each pathogen to insure accuracy of results using the Streak Plate Method. Duncan Multiple Range Test was conducted to determine the root extract that had the highest mean Diameter Zone of Inhibition in each test organism. Results showed that the roots of the six weeds possess antibacterial on the test organism. One Way Analysis of Variance (a0.05) showed that there is no significant difference on the inhibition of the different root extracts. zones of inhibitation of the different root extracts.