Melon Aleyen Grace V. Consular Viary Lycerlene O. Galimpin (March 2013)
Philippine Science High School Western Visayas Campus – Department of Science and Technology (DOST-PSHS WVC), Brgy. Bito-on, Jaro, Iloilo City 5000, Philippines
ABSTRACT
The synergism of Zingiber officinale and Cymbopogon citratus essential oils was determined in this study. The test organism involved the Gram-positive Staphylococcus aureus and gram- negative bacteria Escherichia coll. This study aimed to identify the Minimum Inhibitory Concentration of the individual and combined essential oils and calculate for the Fractional Inhibitory oncentration as a determinant of Synergism. The checkerboard method was used. Thirteen dilutions were done with 5 concentrations ranging from 0.2-0.01 ul. were used for the test. The MIC of individual essential oils after 24 hours were found to range from 0.1 uf. to 0.08 µl and were reduced in the combination of both. The calculated FIC value showed that there is a synergistic activity between the essential oils with the E. coli while an indifference with the S. aureus. Further studies about the key components of the activity of the combination of these essential oils should be done to be able to explain the activity of each essential oils in combination.