SHARI A. ALTAMERA, GIANA MARIE F. FERRER, HANNAH JOYCE L. HALASAN (FEBRUARY 2010)
Philippine Science High School Western Visayas Campus – Department of Science and Technology (DOST-PSHS WVC), Brgy. Bito-on, Jaro, Iloilo City 5000, Philippines
ABSTRACT
Previous studies have reported the presence of antibiotic-resistant Escherichia coli in the intestines of chickens. In this study, E. coli was isolated from the intestines of chickens and was tested against amoxicillin (25µg), ampicillin (25µg), norfoxacin (10µg) and piperacillin (75µg).
Isolation of E. coli was performed using a selective media, Eosin Methylene Blue (EMB) Agar. Disk diffusion method was used to determine the antibiotic susceptibility of E. coli against antibiotics used in the study.
A total of 30 chickens were collected from three different poultry farms. In this study, E. coli was isolated from 29 chicken intestine samples and was tested for antibiotic susceptibility. Highest antibiotic resistance was against piperacillin (96.55%), followed by amoxicillin (82.76%), ampicillin (65.52%) and norfloxacin (24.14%).
The study confirmed the presence of antibiotic-resistant E. coli in the intestines of chickens in poultry farms in Iloilo. Results suggest monitoring the emergence of antibiotic- resistant E. coli in poultry farms, investigating the probable modes of transmission and researching the causes for acquiring resistance of E. coli against antibiotics.