Elma Fe Katrina P. Arboleda Jamie Marie L. Cuadra Paul Lindley B. ‘Leo (March 2013)
Philippine Science High School Western Visayas Campus – Department of Science and Technology (DOST-PSHS WVC), Brgy. Bito-on, Jaro, Iloilo City 5000, Philippines
ABSTRACT
The continued failure of the pharmaceutical industry has led scientists to screen medicinal plants for their antimicrobial potentials (Freile-Pelegrin and Morales 2004, Chong and others 2011). In this study, the antibacterial activity of the hexane and methanol extracts of the Codium bartlettii (green sea antler) from the coast of Brgy. Hoskyn, Jordan, Guimaras was examined against the bacteria (Enterococcus faecalis and Staphylococcus aures) and gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) using the Disk Diffirsion Assay. The antibacterial activity was quamified using the Broth Microdilution for the Minimal Inhibitory Concentration (MIC) and Agar Dilution for the Minimal Bactericidal Concentration (MBC). MIC (MBC/MIC) was computed. The hexane extract exhibited antibacterial activity against the four bacterial strains, while the methanol extracts were only able to inhibit S. aureus. S iureus was the most susceptible to the hexane and methanol extracts with inhibition zones 18mm and 10mm respectively. The MIC value of P. aeruginosa against the hexane extract is 5.00:0.00 mg/ml and the MBC value recorded was 5.00±0.00 mg/ml. The MIC values of the hexane extract against E. fueculis, E. coli, and S. aureus and the methanol extract against S. aureus are greater than 10 mg/ml. The MICde computer was 1.00 and the hexane extract of Codium bartlettil is considered bactericidal (MIC ≤2) against Pseudomonas aeruginosa,