Immune Boosting Activity of Aqueous Lemongrass (Cymbopogon citratus) Leaf Extract on Native Chickens (Gallus gallus domesticus) Challenged with Newcastle Disease

Ella Mae Joy Sira1, Blessy Grace Valzado1, Aris Larroder1 and Jaime Cabarles, Jr.2
1Philippine Science High School – Western Visayas Campus – Bito-on, Jaro, Iloilo City 5000, Department of Science and Technology, Philippines
2Central Philippine University – College of Agriculture, Resources and Environmental Science – Lopez Jaena St, Jaro, Iloilo City, 5000, Philippines


Abstract
Newcastle Disease is a highly infectious disease which can cause up to a 100% mortality in native chickens and no treatment has been yet found for it. Lemongrass (Cymbopogon citratus) is a plant that has alkaloid, saponin, tannin, and flavonoid to combat Newcastle disease. This study aims to measure and compare the antibody titre gain of Philippine native chickens treated with different dilutions of C. citratus aqueous extract. Fofty-four two-month-old chickens were infected with live virus-concentrated solution. Blood was collected from all the samples prior to infection and after the treatment. Samples were grouped into four treatment groups A, B, C, and D, according to the dilution factor. Samples from the treatment group A were given with 1 mL undiluted C. citratus extract, treatment group B with 5-fold dilution, treatment C with 10-fold dilution, and treatment D with 20-fold dilution. Antibody titre gain was determined by hemagglutination inhibition. Result show that there is a significant increase with the antibody titre gain for unvaccinated and vaccinated groups. Unvaccinated samples do not require a treatment diluted in a specific volume of distilled water while vaccinated samples require a treatment diluted in 9 mL distilled water for an optimal antibody increase when infected with ND. Treated samples have an increase in the average live weight with treatments B and C exhibiting a significant increase. Survival rate of the chickens is also high with 85.21%.