ANTIBACTERIAL EFFECTS OF THE CRUDE EXTRACTS OF SOME LOCAL PLANTS ON Pseudomonas aeruginosa AND Bacillus subtilis

Jennifer T. Alabado, Christy P. Daguia, Alpha Lizette B. Viray (February 2000)

Philippine Science High School Western Visayas Campus – Department of Science and Technology (DOST-PSHS WVC), Brgy. Bito-on, Jaro, Iloilo City 5000, Philippines

ABSTRACT

The study was conducted to determine if the crude extracts of achuete seeds, aloe vera leaves, kamantigi leaves, kamoteng-kahoy tuber, oregano leaves, and talong fruit had antibacterial effects on Pseudomonas aeruginosa and Bacillus subtilis. The study was conducted in the Philippine Science High School Research Laboratories. The methodology of the study followed that of the undergraduate thesis of Pranga and Teran (1998). The statistical tools used in this study were the mean and standard deviation for descriptive statistical tools, and the t-test, One Way Analysis of Variance (One Way ANOVA) and Scheffe test, all set at 0.05 alpha level of significance, were used as inferential statistical tools.

The results of this study showed that there were no significant differences in the antibacterial effects of the different extracts in terms of the diameter zones of inhibition they caused upon Pseudomonas aeruginosa and Bacillus subtilis.

The achuete and talong extracts posed no significant differences in the diameter zones of inhibition they caused upon the test organisms compared with water. Therefore, achuete and talong showed antibacterial potential upon Pseudomonas aeruginosa and Bacillus subtilis which was potent as tetracycline. The other extracts from aloe vera, kamantigi, kamoteng-kahoy, and oregano posed significant differences in the antibacterial effect compared with tetracycline and posed no significant difference with water. Therefore, these extracts do not have antibacterial potentials as tetracycline. Furthermore, their antibacterial effects were just like the effect of water.

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