Junette B. Galagala, Damian O. Mansueto III, Francis Rouen T. Pasadilla (March 2000)
Philippine Science High School Western Visayas Campus – Department of Science and Technology (DOST-PSHS WVC), Brgy. Bito-on, Jaro, Iloilo City 5000, Philippines
ABSTRACT
This study was conducted to determine the antibacterial potential of Moringa oleifera leaf oil in different oil-water ratios, namely, 25:75, 50:50 and 75:25, versus distilled water and Tetracycline against Escherichia coli, Salmonella and Staphylococcus aureus. Specifically, the effect of Moringa oleifera oil in the different concentrations on growth of the said bacteria in terms of the zone of inhibition was determined. It was also determined whether there was a significant difference in the antibacterial potential of Moringa oleifera oil in the different oil-water ratios and Tetracycline on the growth of the given bacteria.
It was hypothesized that Moringa oleifera oil has no significant effect on the growth of Escherichia coli, Salmonella and Staphylococcus aureus. It was also hypothesized that there was no significant difference in the antiseptic potency of Moringa oleifera oil in the different concentrations, distilled water and Tetracycline in the zone of inhibition in the growth of the said bacteria.
This study employed the Pretest-Posttest Control Group Research Design. The Control Pretest used distilled water as treatment while the control posttest used the treatments Moringa oleifera oil in oil-water ratios 25:75, 50:50 and 75:25 and Tetracycline.
The statistical tools used in the study were the Mean, the One-Way Analysis of Variance, and the Scheffe test.
The study established that as the Moringa oleifera oil concentration was increased, its potency against Staphylococcus aureus also increased. Moringa oleifera oil in all oil-water concentrations had no effect in Escherichia coli but only Moringa oleifera oil in 25:75 and 50:50 had no effect in Salmonella. Finally, only Moringa oleifera oil in 75:25 had an effect in the Staphylococcus aureus. It was concluded that the effects of Moringa oleifera oil as a whole had a significant difference against Tetracycline and that these effects were not comparable to that of Tetracycline.