Claire Samantha T. Juanito, Darryl Joy C. Juntila, Chicki Florette C. Uy (February 2006)
Philippine Science High School Western Visayas Campus – Department of Science and Technology (DOST-PSHS WVC), Brgy. Bito-on, Jaro, Iloilo City 5000, Philippines
ABSTRACT
Antibacterial potency of fermented molasses against Enterobacter aerogenes, Pseudomanas aeruginosa, Ralstonia solanacearum, and Xanthomonas oryzae pv oryzicola was determined and compared with respect to length of fermentation period and method of preparation. Kirby-Bauer Method was employed to determine the test organisms’ sensitivity to the fermented molasses. Ciprofloxacin was used as positive control and distilled water as negative control. This Trudy aimed to compare the antibacterial effects of fermented molasses prepared at Garin Farm and the researchers-made fermented molasses and to compare the antibacterial activity of the fermented molasses predations to that of the controls. The fermentation procedure used in the study was patterned after Garin Farm’s as to materials and progression of steps. However, quantitative and aseptic techniques were employed in the study. Antibacterial potency of the fermented molasses at 3-, 9-, 15-, 21- and 27- a day fermentation periods were compared. This study demonstrates the fermented molasses possesses antibacterial activity against Enterobacter aerogenes, Pseudomonas aeuroginosa, Ralstoina solanacearum, and Xanthomanas oryzae pv orizycola; Results of paired T-test (a – 0.05) showed that the test organisms were more sensitive to fermented molasses from Garin Farm than that of the researchers. One-Way ANOVA (a=0.05) indicated that length of fermentation period affects the antibacterial potency of Garin Farm fermented molasses on all the test organisms and the antibacterial potency of researchers-made fermented molasses on Enterobacter aerogenes and Xanthomonas oryzae pv orizycola. Multiple Comparison using Scheffe’s Post-Hoc test (a=0.05) revealed that the antibacterial activity of Garin Farm fermented molasses was combatant from 3- to 15- day fermentation period. Antibacterial potency of Garin Farm fermented molasses though less than that of Ciprofloxaci, was greater than that of the researcher-made fermented molasses. Grain Farm fermented molasses is more potent than the researchers-made fermented molasses; and that fermented molasses had greater potency during the early stages of fermentation.