Cuizon, Georgia Mae D. Francisco, Mary Mae Justine B. Payba, Jessamine S. Fourth Year-Tau (March 2008)
Philippine Science High School Western Visayas Campus – Department of Science and Technology (DOST-PSHS WVC), Brgy. Bito-on, Jaro, Iloilo City 5000, Philippines
ABSTRACT
Antibacterial activity of the biab extract added to a liquid detergent soup against Pseudomonas aeruginosa and Staphylococcus aureus was determined and compared with respect to its mean zones of inhibition.
This study aimed to determine the antibacterial activity of the commercial dishwashing liquid, biab methanol extracts, and 1:2 (biab extract dishwashing liquid) biab extract and commercial dish washing liquid mixture in terms of mean zones of inhibition and to compare the antibacterial activity of the commercial dishwashing liquid, biab methanol extracts, and 1:2 (biab extract: dishwashing liquid) biab extract and commercial dish washing liquid mixture.
Kirby-Bauer Dise Diffusion Method was used in the antibacterial bioassay using three replicates for each test organism. Tetracycline was used as a positive control and distilled water as negative control.
The zones of inhibition were measured and compared after a 24 hour incubation period.
Results of this study showed that dishwashing liquid with and without biab extract is more effective compared to biab extract against gram-negative bacterium Pseudomonas aeruginosa. One-Way Analysis of Variance (ANOVA) showed significant antibacterial activity of biab extract and dishwashing liquid with and without biab extract against Pseudomonas aeruginosa.
The antibacterial activity of biab extract is comparable to both dishwashing liquid with biab extract and dishwashing liquid without biab extract against gram-positive bacterium Staphylococcia aureus.
The addition of biab extract to dishwashing liquid showed an increase in the antibacterial acitivity of the dishwashing liquid against both gram-positive and gram-negative bacterium, Pseudomonas aeruginosa and Staphylococcus aureus but the antibacterial activity of dishwashing liquid with biab extract and that of dishwashing liquid without bab extract is comparable against both human pathogens. It could be concluded that the addition of biah extract to commercially available dishwashing liquid does net exhibit a significant change in antibacterial bioactivity.