Mikael Oliver O. Cabasal, Karl Josef F. Legazpi, Gabriel Vilino P. Urethane (March 2012)
Philippine Science High School Western Visayas Campus – Department of Science and Technology (DOST-PSHS WVC), Brgy. Bito-on, Jaro, Iloilo City 5000, Philippines
ABSTRACT
Anacardium Occidentale (cashew) extracts are effective inhibitors of microbial growth. Crude ethanol extracts of cashew leaves can inhibit Pseudomonas aeruginosa bacterial growth at 5000 mL. In the present study, an attempt was made to determine the ability of cashew leaf extracts to inhibit Pseuodmonas aeruginosa bio film formation. Extraction of the leaves was done using a soxhlet apparatus with ethanol as the solvent. Biofilms were developed on PVC strip were then submerged in ethanol to dissolve the crystal-violet stain. Absorbable of ethanol was measured with a wavelength of 570 nm. Biofilm formation with the extract was compared to normal biofilm formation using PVC strip assay in terms of absorbance. The results indicated that the absorbance of the ethanol-dissolved stains was lower in PVC strips with cashew leaf extract. It is concluded that cashew leaf extract has biofilm inhibitory capabilities