Marie Ann A. Libiano, Joyce P. Sodusta, Mark Manfred B. Spitzer (March 2001)
Philippine Science High School Western Visayas Campus – Department of Science and Technology (DOST-PSHS WVC), Brgy. Bito-on, Jaro, Iloilo City 5000, Philippines
ABSTRACT
This study aimed to determine the effects of different substance, i.e., benzene, potassium nitrate and commercial astringent to skin tissue and liver of mice in terms of percentage of damaged cells and mean liver size. It further determined the difference in the percentage of damaged cells and mean liver size of the mice applied with these substances. Four cages were built to shelter the twelve mice in. Benzene, potassium nitrate and commercial astringent were placed in each syringes for their application on the shaven back of the mice. The underlying muscle and the liver were cut, placed in vials and preserved.
Mice treated with benzene had 57.70% damaged cells while those treated with KNO, had 34.02% and those treated with commercial astringent had 64.42%. There was a significant difference in the percentage of damaged cells found in the skin tissues of mice applied with benzene, KNO, commercial astringent, and the control. The mean liver size of mice treated with benzene was 2.60 cm while those treated with potassium nitrate and commercial astringent, were 1.75 cm and 2.44 cm, respectively. There was a significant difference in the mean size of the liver treated with benzene, commercial astringent, potassium nitrate and the control.