Angelica Mia P. Antenor, Rio Portia Loren J. Celiz (March 2014)
Philippine Science High School Western Visayas Campus – Department of Science and Technology (DOST-PSHS WVC), Brgy. Bito-on, Jaro, Iloilo City 5000, Philippine
Abstract
Balbas-pusa is a perennial plant that grows in the Philippines and exhibits a lot of pharmacological activities. In this study, the immune response was compared through measuring and comparing the WBC count with differential of the rats. The initial WBC count with differential of the rats was compared to the WBC count after treatment with vaccine and java tea. Initial blood samples were taken from the saphenous vein and the final blood samples were taken from the arterial vain wherein the rats were euthanized. The blood samples were taken to Medicus Inc. Laboratories for testing. The WBC count of the 2, out of 3, rats that did not take vaccine and tea decreased, and was not able to pass the normal value after 14 days. The lymphocyte count of the 3 rats under the same treatment is normal. The WBC count of the 3 rats that took java tea but were not vaccinated increased and entered the normal range after 14 days. The lymphocyte count of the 3 rats under the same treatment is normal. It was observed that after 14 days, the WBC count of 3 rats that were vaccinated and took java is below the normal value. The lymphocyte count of the same rats decreased and only 1 rat remained normal. The results of this study proved that the saponin in java tea does not affect the immune response of Rattus norvegicus