William Patrick M. Ferolino, Paul O. Legaspi, Jo Maree Pearl J. Oyco (March 2006)
Philippine Science High School Western Visayas Campus – Department of Science and Technology (DOST-PSHS WVC), Brgy. Bito-on, Jaro, Iloilo City 5000, Philippines
ABSTRACT
This study explores the effect of planting Marvelosa in different soil types and sunlight exposure on its medicinal properties. Plants were planted in different soil types and sunlight exposure and harvested when in full-bloom. Extracts were prepared using double decoction. The solvent used for the anti-cancer test extracts was Methylethylketone while the solvent used for the other test extracts was aqueous ethanol. Kirby-Bauer method was used to verify the plants’ anti-bacterial potency. Filter paper discs soaked in extracts were placed in petri plates containing cultures of Staphylococcus aureus. Mean Zones of inhibition (mm) were measured. Yeast Bioassay was used to verify he plants’ anti—cancer activity. Filter paper discs soaked in extracts were placed in petri plates containing cultures of RS 321 and Rad 52 yeast strains. Mean zones of inhibition (mm) were measured. Human blood was placed in different test tubes and mixed with AP extracts. Clotting time was measured when blood was taken from the veins until it becomes jelly-like. Results showed that there is no significant difference in the anti-bacterial and anti-cancer activity of the plant among different soil types and sunlight exposure. However, clotting test showed that extracts from red and sandy loam showed the fastest clotting time of 23.7 and 25.3 mins. One-way Analysis of Variance (p= 0.000, a=0/05) showed that there is a significant difference in the mean clotting time of the blood treated with AP extracts from different soil types. This means that soil type and sunlight exposure affect the plant’s anti-clotting property but not anti-bacterial and anti-cancer potency.